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Mouse Interferon gamma(IFN-γ) ELISA Kit-技术资料/价格-赫澎上海生物
207 人阅读发布时间：2021-07-21 11:08
Mouse Interferon gamma(IFN-γ) ELISA Kit
Catalogue Number: M1519
(96Tests)
Store all reagents at 2-8°C
Collect sample: serum or blood plasma
Assay range ：2.5pg/mL -250pg/mL
FOR LABORATORY RESEARCH USE ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
INTENDED USE
This IFN-γ ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-γ ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PRINCIPLE OF THE ASSAY
The kit assay IFN-γ level in the sample，use Purified antibody to coat microtiter plate wells, make solid-phase antibody, Samples which including standards of known concentrations and unknowns are pipetted into coated microtiter wells, after Incubating ,add Biotinylated anti-IgG,and Combined Streptavidin-HRP, become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution, TMB Chromogen Solution Becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, The intensity of this coloured product is directly proportional to the concentration of IFN-γ present in the samples. measure the optical densit (OD) at 450 nm with microtiter plate reader, calculate IFN-γ concentration by standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. Biotinylated anti-IgG 6.0 mL 1 tube
3. STANDARD(240pg/mL) 0.5ml 1 tube
4. STREPTAVIDIN-HRP 6.0 mL 1 tube
5. STANDARD DILUENT 1.5ml 1 tube
6. Chromogen Solution A 6.0 mL 1 vial
7. Chromogen Solution B 6.0 mL 1 vial
8. STOP SOLUTION 6.0 mL 1 vial
9. WASH SOLUTION x30 20 mL 1 vial
10. Instruction 1
SAMPLE COLLECTION AND STORAGE
Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.
DO NOT USE HEAT-TREATED SPECIMENS.
Catalogue Number: M1519
(96Tests)
Store all reagents at 2-8°C
Collect sample: serum or blood plasma
Assay range ：2.5pg/mL -250pg/mL
FOR LABORATORY RESEARCH USE ONLY.
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
INTENDED USE
This IFN-γ ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of IFN-γ in the sample, this IFN-γ ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus IFN-γ concentration. The concentration of IFN-γ in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PRINCIPLE OF THE ASSAY
The kit assay IFN-γ level in the sample，use Purified antibody to coat microtiter plate wells, make solid-phase antibody, Samples which including standards of known concentrations and unknowns are pipetted into coated microtiter wells, after Incubating ,add Biotinylated anti-IgG,and Combined Streptavidin-HRP, become antibody – antigen - enzyme- antibody complex, after washing Completely, Add TMB substrate solution, TMB Chromogen Solution Becomes blue color At HRP enzyme-catalyzed, And at the effect of acid the color finally become yellow, The intensity of this coloured product is directly proportional to the concentration of IFN-γ present in the samples. measure the optical densit (OD) at 450 nm with microtiter plate reader, calculate IFN-γ concentration by standard curve.
REAGENTS PROVIDED
All reagents provided are stored at 2-8° C. Refer to the expiration date on the label.
1. MICROTITER PLATE 96 wells
2. Biotinylated anti-IgG 6.0 mL 1 tube
3. STANDARD(240pg/mL) 0.5ml 1 tube
4. STREPTAVIDIN-HRP 6.0 mL 1 tube
5. STANDARD DILUENT 1.5ml 1 tube
6. Chromogen Solution A 6.0 mL 1 vial
7. Chromogen Solution B 6.0 mL 1 vial
8. STOP SOLUTION 6.0 mL 1 vial
9. WASH SOLUTION x30 20 mL 1 vial
10. Instruction 1
SAMPLE COLLECTION AND STORAGE
Serum- Use a serum separator tube(SST) and allow samples to clot for 30minutes before centrifugation for 15minutes at approximately 1000 x g.Remove serum and assay immediately or aliquot and store samples at -20 °C or -80°C.
Plasma - Collect plasma using EDTA or heparin as an anticoagulant.Centrifuge samples for 15 minutes at 1000 x g at 2-8°C within 30minutes of collection.Store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
Cell culture fluid and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20°C or -80°C.Avoid repeated freeze-thaw cycles.
NOTE: Serum, plasma, and cell culture fluid samples to be used within 7 days may be stored at 2-8°C, otherwise samples must stored at -20°C(≤2months) or -80°C(≤6months) to avoid loss of bioactivity and contamination. Avoid freeze-thaw cycles .When performing the assay slowly bring samples to room temperature.
DO NOT USE HEAT-TREATED SPECIMENS.